By Sébédio, Jean-Louis
Advances in Conjugated Linoleic Acid study, quantity 2 is the second one e-book in a chain committed completely to conjugated linoleic acid. This ebook has up-to-date details at the research, biochemistry and functions of conjugated fatty acids in an try and make quantity 2, along side quantity 1 (published in 1999), the main complete, up to date assets of CLA-related info to be had this present day. either medical and advertisement perspectives are awarded, with a similar facts occasionally interpreted in a different way
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DMOX derivatives, picolinyl esters, and MTAD adducts have all been used, often in a complementary manner. Before GC-MS analysis, it was necessary to fractionate the metabolites by RP-HPLC, Copyright 2003 by AOCS Press. All rights reserved. primarily to concentrate the low levels of metabolites relative to other fatty acids. Further fractionation by silver-ion HPLC was employed to separate conjugated positional isomers (two 20:3 and two 20:4 isomers) that could not be resolved by GC as methyl esters (79).
42, 1757–1760. 41. , and Murphy, J. (1997) Dietary Influences on Bovine Milk cis-9, trans-11-Conjugated Linoleic Acid Content, J. Food Sci. 62, 1083–1086. 42. , Luedecke. D. (1992) Determination of Conjugated Linoleic Acid Content and Isomer Distribution in Three Cheddar-Type Cheeses: Effects of Cheese Cultures, Processing, and Aging, J. Agric. Food Chem. 40, 1817–1821. Copyright 2003 by AOCS Press. All rights reserved. 43. , and Yamada, K. (1999) A Recommended Esterification Method for Gas Chromatographic Measurement of Conjugated Linoleic Acid, J.
Copyright 2003 by AOCS Press. All rights reserved. Identities of cheese CLA isomers, fractionated by semipreparative silver-ion HPLC using either a single column (8) or two columns in series (3), were confirmed by GC-MS of DMOX derivatives. Twenty isomers (all possible geometrical isomers of 7,9-, 8,10-, 9,11-, 10,12-, 11,13- and 12,14-18:2 except 7c,9t, 8c,10t, 11c,13t and 12t,14c isomers) were identified; the geometry of the double bonds of ct isomers was assigned mainly according to elution order (8).