By Alain Berthod
Micellar Liquid Chromatography studies using surfactant strategies at or above the serious micelle focus as cellular stages in liquid chromatography. It employs a computer-assisted optimization technique and integrates micellar liquid chromatography (MLC) with different chromatographic and electrophoretic thoughts utilizing surfactants. it is also the MICHROM software program package deal on CD-ROM to facilitate the appliance of equations and optimize potency of MLC structures.
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Extra info for Micellar Liquid Chromatography (Chromatographic Science Series)
9 shows the variation in. solubility with t ~ o zwas ~ t described by ionic solided surfactant i later found that ,the micelle structureis an e~uilibriu lar heads andthe a~ractionforces b chains. The tempera~remay affect differently thesetwo comp~ing forces. "h effect of temperature on the cmc values is often complex. At tempera~reshigher than the KraB point, the cmcof most ionicsudactants increases slightly. 0 Temperature dependanceof the SDS solubility. The lSraflEt point of SDS is close to 15°C.
Jandera and J. A , 728: 279 (1996). R. J. Baeza, Description of the Partitioning Behavior of Solutes and Data ~reatmentin Mz;C withModi~ers,Anal. Chim. Acta, 324: I63 (1996). en the surfactant concentration is above a s p ~ c i value: ~ c the c~itical e l k concentratzo~(cmc), the surfactant solution ~ecomesa ~ i c e l l a r chromato~~aphy ( mic~lles, as mobile phases. systems is, thus, required t capabilities of this chromato~raphict e c ~ i q u e . e basic and s ~ p l i ~ e d b a c ~ ~ r o s y s t e ~ sisprovided.
Two examples ofsuch composite surfactants are the alkylethers~~ates: and the carbo~methyletho~ylates~ h aqueous solutions, cationicsudactants are ionized ina cation canyng the amphip~licproperties,and an inactive anion, such as Cl- or Br-. The cationic group is most often a quaternary a ~ o ~ group u m . The general formula is: R’, Ry’and R,”groups can be identical e hydrophobic chain. The . The cetyl or hexadecyl trimethyl onium bromide C ~ ~ H 3 ~ - N ( c HBr3)~, is the cationicsurfactant most studied.