Micellar Liquid Chromatography (Chromatographic Science by Alain Berthod

By Alain Berthod

Micellar Liquid Chromatography studies using surfactant strategies at or above the serious micelle focus as cellular stages in liquid chromatography. It employs a computer-assisted optimization technique and integrates micellar liquid chromatography (MLC) with different chromatographic and electrophoretic thoughts utilizing surfactants. it is also the MICHROM software program package deal on CD-ROM to facilitate the appliance of equations and optimize potency of MLC structures.

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Example text

9 shows the variation in. solubility with t ~ o zwas ~ t described by ionic solided surfactant i later found that ,the micelle structureis an e~uilibriu lar heads andthe a~ractionforces b chains. The tempera~remay affect differently thesetwo comp~ing forces. "h effect of temperature on the cmc values is often complex. At tempera~reshigher than the KraB point, the cmcof most ionicsudactants increases slightly. 0 Temperature dependanceof the SDS solubility. The lSraflEt point of SDS is close to 15°C.

Jandera and J. A , 728: 279 (1996). R. J. Baeza, Description of the Partitioning Behavior of Solutes and Data ~reatmentin Mz;C withModi~ers,Anal. Chim. Acta, 324: I63 (1996). en the surfactant concentration is above a s p ~ c i value: ~ c the c~itical e l k concentratzo~(cmc), the surfactant solution ~ecomesa ~ i c e l l a r chromato~~aphy ( mic~lles, as mobile phases. systems is, thus, required t capabilities of this chromato~raphict e c ~ i q u e . e basic and s ~ p l i ~ e d b a c ~ ~ r o s y s t e ~ sisprovided.

Two examples ofsuch composite surfactants are the alkylethers~~ates: and the carbo~methyletho~ylates~ h aqueous solutions, cationicsudactants are ionized ina cation canyng the amphip~licproperties,and an inactive anion, such as Cl- or Br-. The cationic group is most often a quaternary a ~ o ~ group u m [5]. The general formula is: R’, Ry’and R,”groups can be identical e hydrophobic chain. The . The cetyl or hexadecyl trimethyl onium bromide C ~ ~ H 3 ~ - N ( c HBr3)~, is the cationicsurfactant most studied.

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