By Michael Kinter
How to layout, execute, and interpret experiments for protein sequencing utilizing mass spectrometry
The fast enlargement of searchable protein and DNA databases lately has caused an explosive development within the program of mass spectrometry to protein sequencing. This well timed and authoritative booklet presents execs and scientists in biotechnology learn with whole assurance of techniques for studying protein sequences through mass spectrometry, together with step by step instructions for pattern education, research, and knowledge interpretation.
Michael Kinter and Nicholas Sherman current their very own top quality, laboratory-tested protocols for the research of a large choice of samples, demonstrating how one can perform particular experiments and acquire speedy, trustworthy effects with a ninety nine% luck expense. Readers gets enough experimental element to use of their personal laboratories, know about the right kind choice and operation of tools, and achieve crucial perception into the basic ideas of mass spectrometry and protein sequencing. insurance includes:
- Peptide fragmentation and interpretation of product ion spectra
- Basic polyacrylamide gel electrophoresis
- Preparation of protein digests for sequencing experiments
- Mass spectrometric research utilizing capillary liquid chromatography
- Techniques for protein id by way of database searches
- Characterization of transformed peptides utilizing tandem mass spectrometry
And a lot more
Read Online or Download Protein Sequencing and Identification Using Tandem Mass Spectrometry PDF
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Extra info for Protein Sequencing and Identification Using Tandem Mass Spectrometry
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